anti-syntaxin 4 antibody Search Results


rabbit anti syntaxin4  (Alomone Labs)
94
Alomone Labs rabbit anti syntaxin4
Analysis of Stx4 and Munc18c association in MDA-MB-231 cells. A, Munc18c immunoprecipitates were probed for Munc18c (arrow) and Stx4 (arrow). B+A, beads plus antibody; B+L, beads plus lysate; B+L+IgG, beads plus lysate plus unrelated IgG; IP, immunoprecipitation; Ms, mouse. B, Munc18c immunoprecipitates were probed for Munc18c, Stx4 (arrow), and Stx3 (arrow). C, cells were grown for 4 h on glass coverslips, fixed, permeabilized, and stained for either Munc18c and Stx4 or Munc18c and Stx3. A representative confocal section from the ventral region of a cell is shown. Scale bars = 10 μm. D, analysis of Stx4 and Munc18c association during invadopodium formation (IVF). Cells were seeded onto coverslips coated with PLL or gelatin, incubated for 4 h, lysed, and analyzed by immunoprecipitation/Western blotting. Munc18c immunoprecipitates were probed for Munc18c and Stx4 (arrow). E, quantification of Stx4 co-immunoprecipitated with Munc18c. Percent of controls are from three independent experiments ± S.D. Asterisks denote values significantly different from control unlifted cells (*, p < 0.05). All data represent three or more biological replicates with at least three technical replicates.
Rabbit Anti Syntaxin4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-syntaxin+4+antibody/Anti-Syntaxin+4+Antibody/pmc05625050-402-52-54
Average 94 stars, based on 1 article reviews
rabbit anti syntaxin4 - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
anti-syntaxin 4 antibody  (TargetMol)
N/A
   Buy from Supplier
anti-syntaxin 4 mouse monoclonal antibody clone 49  (Becton Dickinson)
90
Becton Dickinson anti-syntaxin 4 mouse monoclonal antibody clone 49
Analysis of Stx4 and Munc18c association in MDA-MB-231 cells. A, Munc18c immunoprecipitates were probed for Munc18c (arrow) and Stx4 (arrow). B+A, beads plus antibody; B+L, beads plus lysate; B+L+IgG, beads plus lysate plus unrelated IgG; IP, immunoprecipitation; Ms, mouse. B, Munc18c immunoprecipitates were probed for Munc18c, Stx4 (arrow), and Stx3 (arrow). C, cells were grown for 4 h on glass coverslips, fixed, permeabilized, and stained for either Munc18c and Stx4 or Munc18c and Stx3. A representative confocal section from the ventral region of a cell is shown. Scale bars = 10 μm. D, analysis of Stx4 and Munc18c association during invadopodium formation (IVF). Cells were seeded onto coverslips coated with PLL or gelatin, incubated for 4 h, lysed, and analyzed by immunoprecipitation/Western blotting. Munc18c immunoprecipitates were probed for Munc18c and Stx4 (arrow). E, quantification of Stx4 co-immunoprecipitated with Munc18c. Percent of controls are from three independent experiments ± S.D. Asterisks denote values significantly different from control unlifted cells (*, p < 0.05). All data represent three or more biological replicates with at least three technical replicates.
Anti Syntaxin 4 Mouse Monoclonal Antibody Clone 49, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-syntaxin+4+antibody/anti+syntaxin+4+mouse+monoclonal+antibody+clone+49/10__1074_slash_jbc__m611108200-58-0-13
Average 90 stars, based on 1 article reviews
anti-syntaxin 4 mouse monoclonal antibody clone 49 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
mouse monoclonal antibody syntaxin-4  (Becton Dickinson)
90
Becton Dickinson mouse monoclonal antibody syntaxin-4
Analysis of Stx4 and Munc18c association in MDA-MB-231 cells. A, Munc18c immunoprecipitates were probed for Munc18c (arrow) and Stx4 (arrow). B+A, beads plus antibody; B+L, beads plus lysate; B+L+IgG, beads plus lysate plus unrelated IgG; IP, immunoprecipitation; Ms, mouse. B, Munc18c immunoprecipitates were probed for Munc18c, Stx4 (arrow), and Stx3 (arrow). C, cells were grown for 4 h on glass coverslips, fixed, permeabilized, and stained for either Munc18c and Stx4 or Munc18c and Stx3. A representative confocal section from the ventral region of a cell is shown. Scale bars = 10 μm. D, analysis of Stx4 and Munc18c association during invadopodium formation (IVF). Cells were seeded onto coverslips coated with PLL or gelatin, incubated for 4 h, lysed, and analyzed by immunoprecipitation/Western blotting. Munc18c immunoprecipitates were probed for Munc18c and Stx4 (arrow). E, quantification of Stx4 co-immunoprecipitated with Munc18c. Percent of controls are from three independent experiments ± S.D. Asterisks denote values significantly different from control unlifted cells (*, p < 0.05). All data represent three or more biological replicates with at least three technical replicates.
Mouse Monoclonal Antibody Syntaxin 4, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-syntaxin+4+antibody/anti+syntaxin+4+mouse+monoclonal+antibody/pmc02846406-513-4-7
Average 90 stars, based on 1 article reviews
mouse monoclonal antibody syntaxin-4 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
anti-syntaxin 4 rabbit monoclonal antibody  (Boster Bio)
N/A
Boster Bio Anti-Syntaxin 4 Rabbit Monoclonal Antibody catalog # M05345. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
   Buy from Supplier
anti-syntaxin 4 antibody - carrier free  (Alomone Labs)
N/A
Anti-Syntaxin 4 Antibody - (Carrier Free) (#ANR-004-CF). This ready-to-use, carrier-free formulation is free of BSA and sodium azide, making it ideal for conjugation, sensitive downstream applications and specialized assay development. The size of this version
   Buy from Supplier

Image Search Results


Analysis of Stx4 and Munc18c association in MDA-MB-231 cells. A, Munc18c immunoprecipitates were probed for Munc18c (arrow) and Stx4 (arrow). B+A, beads plus antibody; B+L, beads plus lysate; B+L+IgG, beads plus lysate plus unrelated IgG; IP, immunoprecipitation; Ms, mouse. B, Munc18c immunoprecipitates were probed for Munc18c, Stx4 (arrow), and Stx3 (arrow). C, cells were grown for 4 h on glass coverslips, fixed, permeabilized, and stained for either Munc18c and Stx4 or Munc18c and Stx3. A representative confocal section from the ventral region of a cell is shown. Scale bars = 10 μm. D, analysis of Stx4 and Munc18c association during invadopodium formation (IVF). Cells were seeded onto coverslips coated with PLL or gelatin, incubated for 4 h, lysed, and analyzed by immunoprecipitation/Western blotting. Munc18c immunoprecipitates were probed for Munc18c and Stx4 (arrow). E, quantification of Stx4 co-immunoprecipitated with Munc18c. Percent of controls are from three independent experiments ± S.D. Asterisks denote values significantly different from control unlifted cells (*, p < 0.05). All data represent three or more biological replicates with at least three technical replicates.

Journal: The Journal of Biological Chemistry

Article Title: Interaction of Munc18c and syntaxin4 facilitates invadopodium formation and extracellular matrix invasion of tumor cells

doi: 10.1074/jbc.M117.807438

Figure Lengend Snippet: Analysis of Stx4 and Munc18c association in MDA-MB-231 cells. A, Munc18c immunoprecipitates were probed for Munc18c (arrow) and Stx4 (arrow). B+A, beads plus antibody; B+L, beads plus lysate; B+L+IgG, beads plus lysate plus unrelated IgG; IP, immunoprecipitation; Ms, mouse. B, Munc18c immunoprecipitates were probed for Munc18c, Stx4 (arrow), and Stx3 (arrow). C, cells were grown for 4 h on glass coverslips, fixed, permeabilized, and stained for either Munc18c and Stx4 or Munc18c and Stx3. A representative confocal section from the ventral region of a cell is shown. Scale bars = 10 μm. D, analysis of Stx4 and Munc18c association during invadopodium formation (IVF). Cells were seeded onto coverslips coated with PLL or gelatin, incubated for 4 h, lysed, and analyzed by immunoprecipitation/Western blotting. Munc18c immunoprecipitates were probed for Munc18c and Stx4 (arrow). E, quantification of Stx4 co-immunoprecipitated with Munc18c. Percent of controls are from three independent experiments ± S.D. Asterisks denote values significantly different from control unlifted cells (*, p < 0.05). All data represent three or more biological replicates with at least three technical replicates.

Article Snippet: Antibodies were purchased from the following suppliers: rabbit anti-VAMP2 (Affinity Bioreagents, PA1 766); rabbit anti-MMP14, mouse anti-MMP14, rabbit anti-GFP, and rabbit anti-Munc18c (Abcam, ab3644, ab78738, ab290, and ab175238, respectively); mouse anti-Munc18c, mouse anti-SNAP23, mouse anti-syntaxin3, and rabbit anti-EGFR (Santa Cruz Biotechnology, sc-373813, sc-166244, sc-393518, and sc-03, respectively); mouse anti-syntaxin4 (BD Biosciences, 610439); rabbit anti-syntaxin4 (Alomone Labs, ANR-004); mouse anti-actin (Pierce, MA5-15739); mouse anti-β 1 integrin, mouse anti-β-tubulin, and mouse anti-GAPDH (Developmental Hybridoma Studies Bank, P4C10, E7-s, and DSHB-hGAPDH-2G7, respectively); and mouse anti-FLAG and mouse anti β-actin, (Sigma-Aldrich, F3165 and A2228, respectively).

Techniques: Analysis, Immunoprecipitation, Glass, Staining, Confocal, Incubation, Western Blot, Control

RNAi-mediated knockdown of Munc18c impairs invadopodium formation. A, distribution of Munc18c during the formation of invadopodia. Cells were analyzed by confocal microscopy after being plated on Alexa Fluor 594–labeled gelatin for 4 h, fixed, permeabilized, and stained as indicated: β1 integrin and Stx4 (cyan, white arrows), Munc18c (green, yellow arrows). Dark spots in the gelatin field indicate sites of gelatin degradation corresponding to invadopodia. B, cells were transfected with siRNA targeting Munc18c or nonspecific control siRNA, lysed, and analyzed for Munc18c by SDS-PAGE/Western blotting. C, quantification of Munc18c knockdown normalized to actin. D, cells with F-actin puncta overlying dark spots of gelatin degradation were counted as cells forming invadopodia. Shown are percentages of cells forming invadopodia, normalized to control (GFP-transfected) cells. E, invadopodium-based degradation of Alexa Fluor 594 gelatin by cells co-transfected with GFP and control siRNA or Munc18c siRNA. Cells were transfected for 44 h, seeded onto gelatin for 4 h, and then fixed, permeabilized, stained for F-actin, and analyzed by confocal microscopy. Percent of controls are from three independent experiments ± S.D. Asterisks denotes values significantly different from control cells (*, p < 0.05). Scale bars = 10 μm. All data represent three or more biological replicates with at least three technical replicates.

Journal: The Journal of Biological Chemistry

Article Title: Interaction of Munc18c and syntaxin4 facilitates invadopodium formation and extracellular matrix invasion of tumor cells

doi: 10.1074/jbc.M117.807438

Figure Lengend Snippet: RNAi-mediated knockdown of Munc18c impairs invadopodium formation. A, distribution of Munc18c during the formation of invadopodia. Cells were analyzed by confocal microscopy after being plated on Alexa Fluor 594–labeled gelatin for 4 h, fixed, permeabilized, and stained as indicated: β1 integrin and Stx4 (cyan, white arrows), Munc18c (green, yellow arrows). Dark spots in the gelatin field indicate sites of gelatin degradation corresponding to invadopodia. B, cells were transfected with siRNA targeting Munc18c or nonspecific control siRNA, lysed, and analyzed for Munc18c by SDS-PAGE/Western blotting. C, quantification of Munc18c knockdown normalized to actin. D, cells with F-actin puncta overlying dark spots of gelatin degradation were counted as cells forming invadopodia. Shown are percentages of cells forming invadopodia, normalized to control (GFP-transfected) cells. E, invadopodium-based degradation of Alexa Fluor 594 gelatin by cells co-transfected with GFP and control siRNA or Munc18c siRNA. Cells were transfected for 44 h, seeded onto gelatin for 4 h, and then fixed, permeabilized, stained for F-actin, and analyzed by confocal microscopy. Percent of controls are from three independent experiments ± S.D. Asterisks denotes values significantly different from control cells (*, p < 0.05). Scale bars = 10 μm. All data represent three or more biological replicates with at least three technical replicates.

Article Snippet: Antibodies were purchased from the following suppliers: rabbit anti-VAMP2 (Affinity Bioreagents, PA1 766); rabbit anti-MMP14, mouse anti-MMP14, rabbit anti-GFP, and rabbit anti-Munc18c (Abcam, ab3644, ab78738, ab290, and ab175238, respectively); mouse anti-Munc18c, mouse anti-SNAP23, mouse anti-syntaxin3, and rabbit anti-EGFR (Santa Cruz Biotechnology, sc-373813, sc-166244, sc-393518, and sc-03, respectively); mouse anti-syntaxin4 (BD Biosciences, 610439); rabbit anti-syntaxin4 (Alomone Labs, ANR-004); mouse anti-actin (Pierce, MA5-15739); mouse anti-β 1 integrin, mouse anti-β-tubulin, and mouse anti-GAPDH (Developmental Hybridoma Studies Bank, P4C10, E7-s, and DSHB-hGAPDH-2G7, respectively); and mouse anti-FLAG and mouse anti β-actin, (Sigma-Aldrich, F3165 and A2228, respectively).

Techniques: Knockdown, Confocal, Microscopy, Labeling, Staining, Transfection, Control, SDS Page, Western Blot